pe conjugated igg subtype specific mab clones igg1  (Jackson Immuno)

Journal: The Journal of Cell Biology

Article Title: A global chromatin compaction pathway that represses germline gene expression during starvation

doi: 10.1083/jcb.202009197

Figure Lengend Snippet: Widespread H3K9 methylation is observed as chromatin compaction starts in C. elegans PGCs. (A) C. elegans embryos at different embryonic stages were fixed and stained for P-granules (green), H3K9me3 (red), and DNA (blue). Representative whole-embryo images are shown. (B) Same as A except only Z2/Z3 (germ) or a neighboring somatic cell (soma) is shown. The developmental stage of the embryo from which the image was taken is indicated ( n = 20). See for a summary of signal intensities. Scale bar represents a length of 2 µm.

Article Snippet: H3K9me2: Mouse antibody (subtype: IgG1) from Molecular and Biological Laboratories (MABI0317; MBL) was used at 1:1,000.

Techniques: Methylation, Staining

Journal: The Journal of Cell Biology

Article Title: A global chromatin compaction pathway that represses germline gene expression during starvation

doi: 10.1083/jcb.202009197

Figure Lengend Snippet: A TOP-2/condensin II–dependent increase in heterochromatin marks coincides with chromatin compaction in Z2/Z3. (A) Wild-type starved L1s and starved L1s that were defective for methyltransferases (mutants for met-2 , set-25 , set-32, and F1s from animals treated with met-2/set-25 double RNAi) were fixed and stained for P-granules (white), H3K9me2 (green), H3K9me3 (red), and DNA (blue). ( n = 40). See for a summary of signal intensities. (B) L1s were either starved or fed for a varying amount of time. Samples were then fixed and stained for P-granules (white), H3K9me3 (red), H3K9me2 (green), and DNA (blue). Representative images are shown. ( n = 40). See for a summary of signal intensities. (C) Starved L1s, born from strain N2 treated with either control RNAi or capg-2 RNAi, were fixed and stained for P-granules (white), H3K9me2 (green), H3K9me3 (red), and DNA (blue). Representative images are shown ( n = 40). See for a summary of signal intensities. (D) Starved L1s, born from strain N2 treated with either control or top-2 RNAi, were fixed and stained for H3K9me3 (red) and DNA (blue). Representative images are shown ( n = 40). See for a summary of signal intensities. (E) Worms that express HPL-2::mKate were optionally treated with control and capg-2 RNAi. Live embryos were extracted and were imaged for HPL-2 signal. The white star identifies Z2/Z3. Representative images are shown ( n = 20). (F) Quantification of HPL-2::mKate signal from the images taken in E. Error bars represent one standard deviation. Scale bar represents a length of 2 µm.

Article Snippet: H3K9me2: Mouse antibody (subtype: IgG1) from Molecular and Biological Laboratories (MABI0317; MBL) was used at 1:1,000.

Techniques: Staining, Control, Standard Deviation

Journal: The Journal of Cell Biology

Article Title: A global chromatin compaction pathway that represses germline gene expression during starvation

doi: 10.1083/jcb.202009197

Figure Lengend Snippet: Both H3K9 methyltransferases, MET-2 and SET-25, are needed for chromatin compaction in starved L1s. New and starved L1s born from strain WMM1 treated with either control RNAi, met-2 RNAi, or set-25 RNAi or met-2/set-25 double RNAi were used. Z2/Z3 from the L1s were imaged for chromatin compaction. Representative images are shown. Compaction was also quantified and shown below images. Error bars represent one standard deviation ( n = 20). Scale bar represents a length of 2 µm.

Article Snippet: H3K9me2: Mouse antibody (subtype: IgG1) from Molecular and Biological Laboratories (MABI0317; MBL) was used at 1:1,000.

Techniques: Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Berberine Delays Onset of Collagen-Induced Arthritis through T Cell Suppression

doi: 10.3390/ijms22073522

Figure Lengend Snippet: The effect of berberine on circulating anti-bovine type II collagen (CII) IgG in the CIA model. ( A ) Anti-CII IgG1, IgG2a, and total IgG at day 28 among all mice (arthritic and non-arthritic) within BBR, PBS (vehicle control), CIA (no treatment control), and non-CIA control animals ( n = 10 per group). ( B ) Anti-CII IgG levels at day 28 compared among arthritic mice only (BBR n = 5; PBS n = 9; CIA n = 9). Statistical comparisons made with the Kruskal–Wallis test with Dunn’s multiple comparisons. ( C ) Anti-CII IgG levels at day 28 compared among BBR-treated mice who developed arthritis (“arthritic”) vs. those that did not (“non-arthritic”). Statistical comparisons made with the Mann–Whitney U test. For all statistical tests in A–C, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Serum concentrations of anti-collagen type II (anti-CII) total IgG (catalog # 1012T), anti-CII IgG1 (catalog # 20321T), and anti-CII IgG2a (catalog # 20322T) were measured by ELISA (Chondrex, Redmond, WA, USA) according to the manufacturer’s instructions [ , ].

Techniques: MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: PLD1 deficiency suppresses the collagen type II-specific humoral response and the production of proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen total IgG, IgG1, and IgG2a antibodies was measured by ELISA in the serum from the indicated mice at day 42. ( B ) Measurement of proinflammatory cytokines in the serum of the indicated mice at day 42 as analyzed by ELISA. n = 15 per group. * p < 0.05, N.S., non-significant. Results are shown as mean ± standard error of the mean (SEM).

Article Snippet: The amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: The PLD1 inhibitor reduces the production of the anti-CII IgG2a autoantibody and proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen IgG1 and IgG2a antibodies was measured by ELISA in the serum of the indicated mice at day 45. ( B ) The amount of proinflammatory cytokines was measured by ELISA in the serum of the indicated mice at day 45. n = 15 per group. * p < 0.05. Results are shown as mean ± standard error of the mean (SEM).

Article Snippet: The amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay